Learn more.Ĭellular Imaging and Analysis (i.e., SNAP and CLIP products) However, this research should always be done in safe and ethical manner. New England Biolabs (NEB) is committed to practicing ethical science - we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in.
This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals. While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.įor more information about commercial rights, please email us at product is intended for research purposes only. This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). Figure 1: Structure of CLIP-Biotin (MW 569.7 g/mol) The labeling of fusion proteins with CLIP-Biotin is described below. Expression of CLIP-tag fusion proteins is described in the documentation supplied with CLIP-tag plasmids. There are two steps to using this system: subcloning and expression of the protein of interest as a CLIP-tag fusion, and labeling of the fusion with the CLIP-tag substrate of choice. CLIP-tag and SNAP-tag can be used for orthogonal simultaneous labeling. Although CLIP-tag is based on the same protein as SNAP-tag ®, the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag.
In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether link. CLIP-tag substrates are derivatives of benzylcytosine (BC). CLIP-tag is a small protein based on mammalian O 6-alkylguanine-DNA-alkyltransferase (AGT). This package contains 50 nmol of CLIP-Biotin substrate, sufficient to make 10 ml of a 5 μM solution for the labeling of CLIP-tag fusion proteins in cells.ĬLIP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest.
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